Candice S. Klug, PhD

James S. Hyde Professor of Biophysics; Director, National Biomedical EPR Center; Program Director, Biophysics Graduate Program

Locations

Biophysics
MFRC 2026

Contact Information

Education

Postdoctoral Fellow in Ophthalmic Biophysical Chemistry, UCLA School of Medicine, Jules Stein Eye Institute, Los Angeles, CA, 1999-2001
PhD, Biophysics, 果冻影院, Milwaukee, WI, 1999
BS, Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 1994

Biography

I received my BS in chemistry from Massachusetts Institute of Technology and my PhD in biophysics from 果冻影院. I completed my postdoctoral training in ophthalmic biophysical chemistry at the UCLA Jules Stein Eye Institute. I returned to the 果冻影院 Department of Biophysics as faculty in 2001 and am currently the James S. Hyde Professor of Biophysics. I have been funded by the NIH my entire career.

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                    Methodologies and Techniques
                

                    Adenosine Triphosphatases
Amino Acid Motifs
ArnT
Arrestin
Arrestins
ATP-Binding Cassette Transporters
Bacterial Proteins
electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
Escherichia coli Proteins
Lipid A
Lipopolysaccharides

            

                
                    Leadership Positions
                

                    Acting Director, IDP Program in Biomedical Sciences, 2019-2020
Associate Director, National Biomedical EPR Center, 2014-2016
Chair, Graduate School Curriculum and Programs Committee, 2010-2013
Chair, Women in Science Advisory Committee, 2011 - 2016
Chair, Women's Faculty Council, 2006-2007
Director, National Biomedical EPR Center, 2016 - present
President, 果冻影院 Faculty Council, 2016-2017
Program Director, Biophysics Graduate Program, 2013 - present
Training Program Director, National Biomedical EPR Center, 2003-present 

            

                
                    果冻影院 Program / Core Facilities
                

                    Biophysics Graduate Program
Interdisciplinary Program in the Biomedical Sciences
National Biomedical EPR Center

            

Research Interests

My lab studies the functional dynamics of both soluble and membrane proteins, with a specific focus on bacterial proteins involved in lipid transport using electron paramagnetic resonance (EPR) spectroscopy, as well as EPR technology development.

Lpt Protein-Mediated Transport of LPS is an R01 award from the National Institute of General Medical Sciences. The goal of this project is to gain insights into the mechanism of LPS transport across the periplasm of Gram-negative bacteria using site-directed spin labeling EPR spectroscopy, complementary biophysical approaches and in vivo growth assays to enable rational antibiotic drug design.

Conformation and Functional Dynamics of a Bacterial PASTA Kinase is a multi-PI (with Dr. Christopher J. Kristich) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to study the conformation and orientation of the intact IreK PASTA module in solution and in full-length IreK embedded in a membrane and elucidate the dynamic changes in the kinase domain associated with kinase activation using a complementary combination of in vivo functional analyses and biophysical approaches.

Development of High-Throughput, High-Sensitivity EPR Sample Handling Capabilities for Biomedical Research is a multi-PI (with Dr. Michael Lerch) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to develop two innovative EPR spectrometer technologies with outstanding sample sensitivity that are easy to use and widely available to the scientific community.

Lab Members
Nicholas Cina, Graduate Student
Kathryn Schultz, Research Scientist I

(Cina NP, Klug CS.) Appl Magn Reson. 2024 Mar;55(1-3):141-158 PMID: 38645307 PMCID: PMC11025719 04/22/2024
(Cina NP, Frank DW, Klug CS.) Protein Sci. 2024 Feb;33(2):e4879 PMID: 38131105 PMCID: PMC10804673 SCOPUS ID: 2-s2.0-85182824310 12/22/2023
(Cina NP, Klug CS.) Applied Magnetic Resonance. March 2024;55(1-3):141-158 SCOPUS ID: 2-s2.0-85166977110 03/01/2024
(Yang Z, Klug C, Kim M.) Applied Magnetic Resonance. March 2024;55(1-3):1-4 SCOPUS ID: 2-s2.0-85181210257 03/01/2024
(VanZeeland NE, Schultz KM, Klug CS, Kristich CJ.) J Mol Biol. 2023 Sep 15;435(18):168216 PMID: 37517789 PMCID: PMC10528945 SCOPUS ID: 2-s2.0-85166951228 07/31/2023
(Schultz KM, Schneider JR, Fischer MA, Cina NP, Riegert MO, Frank DW, Klug CS.) Protein Sci. 2023 Aug;32(8):e4724 PMID: 37417889 PMCID: PMC10360375 SCOPUS ID: 2-s2.0-85165512022 07/07/2023
(Bluma MS, Schultz KM, Kristich CJ, Klug CS.) Protein Sci. 2023 Jul;32(7):e4697 PMID: 37312631 PMCID: PMC10303680 SCOPUS ID: 2-s2.0-85163810164 06/14/2023
(Perry-Hauser NA, Hopkins JB, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Chung KY, Klug CS, Gurevich VV, Iverson TM.) J Mol Biol. 2022 Apr 15;434(7):167465 PMID: 35077767 PMCID: PMC8977243 SCOPUS ID: 2-s2.0-85124130291 01/26/2022
(Schultz KM, Klug CS.) Methods Mol Biol. 2022;2548:83-96 PMID: 36151493 PMCID: PMC10765611 SCOPUS ID: 2-s2.0-85138460647 09/24/2022
(Chen Q, Zhuo Y, Sharma P, Perez I, Francis DJ, Chakravarthy S, Vishnivetskiy SA, Berndt S, Hanson SM, Zhan X, Brooks EK, Altenbach C, Hubbell WL, Klug CS, Iverson TM, Gurevich VV.) J Mol Biol. 2021 Feb 19;433(4):166790 PMID: 33387531 PMCID: PMC7870585 SCOPUS ID: 2-s2.0-85100159529 01/03/2021
(Klug, C.S., Lerch, M.T., Feix, J.B. .) Nitroxides: Synthesis, Properties, and Applications. Klug, C.S., Lerch, M.T., Feix, J.B. (2021) Applications of nitroxide spin labels to structural biology in: Nitroxides: Synthesis, Properties, and Applications. (Ouari, O., Gigmes, D., eds.) Royal Society of Chemistry, pp. 392-419. 05/14/2021
(Zhuo Y, Gurevich VV, Vishnivetskiy SA, Klug CS, Marchese A.) J Biol Chem. 2020 Oct 09;295(41):14111-14124 PMID: 32753481 PMCID: PMC7549033 SCOPUS ID: 2-s2.0-85092803357 08/06/2020

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